NASC

NASC ID: N632865

Name: SALK_132865

ABRC stock number: SALK_132865

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g46280 F12M12. 25 0 R300-5' F12M12. 25 0 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_132865.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G46280.1 (at chr 3 pos 17008485 (W/17008414-17008485) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 34, R 39, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347144.

SALKseq_132865.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G14670.1=UTR3 and AT1G14680.1 (at chr 1 pos 5040681 (W/5040640-5040681) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 50-78 bps onto the T-DNA. Pools P 34, R 39, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343801.

SALKseq_132865.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G10520.1 and AT4G10530.1 (at chr 4 pos 6507504 (C/6507504-6507552) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 34, R 39, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350491.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_132865

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664037 (SALK_132865C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G14670 AT1G14680 AT4G10520 AT4G10530 At3g46280

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.