NASC

NASC ID: N586765

Name: SALK_086765

ABRC stock number: SALK_086765

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At2g34970. F19I3.20 R300-3' At2g34980 F19I3.21 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_086765.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G34970.1 (at chr 2 pos 14748727 (W/14748669-14748727) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 79, R 80, C 73 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438344.

SALKseq_086765.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G73030.1 (at chr 1 pos 27474933 (W/27474892-27474933) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 79, R 80, C 73 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435040.

SALKseq_086765.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G02230.1 (at chr 2 pos 591843 (C/591843-591905) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 56-76 bps onto the T-DNA. Pools P 79, R 80, C 73 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418471.

SALKseq_086765.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G02440.1 and AT2G02450.1 (at chr 2 pos 643749 (C/643749-643796) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 79, R 80, C 73 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421787.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_086765

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663192 (SALK_086765C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G73030 AT2G02230 AT2G02440 AT2G02450 At2g34970

EMBL accession:

BH855010

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.