NASC Stock Detail Page
NASC ID: N8122
Name: Ds Cre-lox line
ABRC stock number: CS8122
Description: transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to 12s; stocks provided by C. Corr, B. Osborne, and B. Baker.
Donation Date: 1996-06-20
Donated by: | The Plant Gene Expression Center Barbara Baker The Plant Gene Expression Center Cathy Corr The Plant Gene Expression Center Brian Osborne |
Donor Number: D56 (R)
Part of : Baker Ds Cre-lox
Stock type: individual line
Material type: seed
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Seed type: Transposon insertion
Mutagen: Ds Transposon Insertion
Background: No-0(N3081)
Transformation method: Agrobacterium transformation
References
Osborne, B.I., Wirtz, U. & Baker, B. 1995. A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. The Plant Journal 7(4):687-701.PubMed ID: 7742862.