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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N77195

Name: Puk-2

ABRC stock number: CS77195

Description: Natural accession donated by the J. Bergelson laboratory at the University of Chicago - USA as part of the 1001 Genomes Project. More detailed information can be found at http://1001genomes.org. Bulk seed were collected from offspring of a single sequenced plant.

Donation Date: 2008-11-14

Donated by:  University of Chicago Joy Bergelson

Donor Number: 9437

Part of : 1001 Genomes Project

Stock type: individual line

Material type: seed

Comment: Stock current unavailable due to validation problem. Waiting for new donation.

Status Price (£)
Available £7.50

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Ecotype

Background: Puk-2

Segregation status: unknown

Growth requirement: These accessions spend most of their natural lives at temperatures below 10C. Vernalization is required to promote flowering if they are grown at higher temperatures (i.e. 22-23C). Recommended growth conditions: one week of stratification at 4C to overcome seed dormancy and accomplish optimal germination, followed by 70-160 days of growth at 10C to induce flowering under long day conditions (16h light/8h dark). Alternative growth conditions: one week of stratification├┐at 4C to overcome seed dormancy and accomplish optimal germination, followed by two weeks of growth at 22-23C, 8-10 weeks of vernalization at 4C and 10-40 days at 22-23C to induce flowering under long day conditions (16h light/8h dark).

Ecotype data

Location: Pukavik Bus

Country: SWEDEN

Latitude Min: 56.1633

Latitude Max: 56.1633

Longitude Min: 14.6806

Longitude Max: 14.6806

References

Long et al. 2013. Massive genomic variation and strong selection in Arabidopsis thaliana lines from Sweden. Nature genetics 45(8):884-90. PubMed ID: 23793030.