NASC

Donated by

• Thomas Mitchell-Olds Department of Bioorganic Chemistry, Max-Planck-Institut für Chemische Ökologie
• Juergen Kroymann Department of Genetics and Evolution, Max-Planck-Institut für Chemische Ökologie

Click here to view all 202 of these lines.

Description

Generation of Da(1)-12×Ei-2 Recombinant Inbred Lines

This recombinant inbred population was produced by the Kroymann group by crossing the ecotypes Da (1)-12 and Ei -2. Da(1)-12 (accession no. N917) and Ei-2 (N1124) accessions were obtained from the Arabidopsis stock center (Nottingham, U.K.). Except for the initial cross between both accessions, all following generations were propagated by selfing. 215 F2 plants were randomly selected from the progeny of a single heterozygous F1 plant. For every advanced generation, 4–8 seeds per line were planted, and a single plant was randomly chosen from each line for seed production. Finally, seeds from a single F8 plant per line were bulk-collected resulting in a final set of 202 Da(1)-12×Ei-2 RILs.

Genotyping and genetic map

Molecular markers were obtained from publicly available sources, or were generated from microsatellite loci identified in the Col-0 genome sequence. More than 150 potential markers were tested for polymorphism between parental lines, using DNA from Da(1)-12, Ei-2, and a 1:1 mixture of DNAs from both lines. 84 PCR products were found suitable for genotyping, and allowed to distinguish between Da(1)-12 and Ei-2 genotypes, and between homozygous and heterozygous loci. Genotyping was carried out with DNA extracted from individual F9 progeny. Genotype data for the Da(1)-12×Ei-2 RILs is available in the publication below.

Further information about the population

Further information about this population can be found in the publication Pfalz M, et .al (2007).

Ordering the population

The RI lines can be ordered individually or as a complete set of 202 individual lines (N722034)

Nasc code	Description	Stock contents
N722034	Da(1)-12 x Ei-2 recombinant inbred set containing 202 lines (N721832...N722033)	View set contents

References

• Pfalz, M. et al. 2007. Mapping of QTL for Resistance against the Crucifer Specialist Herbivore Pieris brassicae in a New Arabidopsis Inbred Line Population, Da(1)-12 x Ei-2 . PLoS ONE 2(6):e578. PMID. 17593977.