Van Lijsebettens T-DNA
Donated by
- Mieke van Lijsebettens Chromatin and Growth Group, VIB Department of Plant Systems Biology, Faculty of Sciences, Ghent University
Click here to view all 8 of these lines.
Description
Van Lijsebettens T-DNA
- Contains T-DNA insertion with dominant marker which can be used for efficient gene mapping; markers associated with T-DNA - hpt (hygromycin phosphotransferase, hygromycin resistance), neo (neomycin phosphotransferase, kanamycin resistance), gus (beta-glucuronidase).
- No phenotype information available at this time
Marker Lines with Mapped T-DNA Insertions from Dr. Mieke van Lijsebettens
These stocks, donated by Dr. M. Van Lijsebettens, Ghent, Belgium, contain T-DNA insertions having at least one dominant marker. These can be used to increase the efficiency of gene mapping by recombinational methods. See cited publication for details of usage.
| Nascode | Donor Name | Map pos. | Background | T-DNA Markers* | Allele symbol/ name | Visible phenotype |
| N5208 | TDNA1 | 1-32 | C24 | neo, bar | pfl-1, gl1/ointed first leaf, glabra | Insertion is in pointed first leaf locus; pfl: first true leaf is narrow, pointed and chlorotic;ubsequent leaves are pointed but less chlorotic; fertile at 13C; growth is reduced at 20C; reduced trichomes. |
| N5209 | TDNA2 | 1-67 | C24 | neo, bar | gl1/glabra | reduced trichomes. |
| N5210 | TDNA3 | 2-60 | Col | hpt, gus | - | |
| N5211 | TDNA4 | 3-43 | C24 | neo, bar | gl1/glabra | reduced trichomes. |
| N5212 | TDNA5 | 5-25 | C24 | hpt | gl1/glabra | reduced trichomes. |
| N5213 | TDNA6 | 5-72 | C24 | hpt, iaaH | gl1/glabra | reduced trichomes. |
| N5214 | TDNA7 | 5-84 | C24 | neo, bar | gl1/glabra | reduced trichomes. |
| N5215 | TDNA8 | 5-81 | C24 | bar, htp, Ds | gl1/glabra | reduced trichomes. |
* neo = neomycin phosphotransferase, kanamycin resistance
bar = phosphonothricin resistance
hpt = hygromycin phosphotransferase, hygromycin resistance
gus = beta-glucuronidase
iaaH = indole-3-acetamide, susceptibility to auxin conjugates
Ds = maize dissociation transposable element.
References
- Van Lijsebettens, M. et al. 1996. Transgenic Arabidopsis tester lines with dominant marker genes. Molecular & general genetics 251(3):365-72.PubMed ID: 8676880.